Mayer’s Hematoxylin (Lillie’s Modification) is a progressive nuclear hematoxylin stain with several histological applications. Nuclei should staining is strong, clean, crisp, and no differentiation is needed. Bluing reagent (Catalog: BRT) may be used following hematoxylin to “blue” or alter the shade of hematoxylin from a purple to blue. Nuclei: Purple. Nuclei after Bluing: Blue. Blue nuclei after Eosin: Blue to Violet.
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Hematoxylin is a nuclear stain that is used as a counterstain for immunohistochemistry (IHC). Together with eosin, it forms the Hematoxylin and Eosin stain (H&E stain), one of the most commonly used stains in histology.
Summary Protocol:
Apply Hematoxylin to completely cover specimen and incubate for 2-10 minutes with hematoxylin according to desired intensity.
